Copyright © 2014 Sociedade Brasileira de Anestesiologia. Postoperative epidural catheter analgesia, under this study conditions, was found to be low risk for bacterial colonization in patients at surgical wards. Microorganisms isolated from the catheter tip were Staphylococcus aureus, Pseudomonas aeruginosa and Sphingomonas paucimobilis. The type of surgery (contaminated or uncontaminated), physical status of patients, and surgical time showed no relation with the colonization of catheters. No patient had superficial or deep infection. Of 68 cultured catheters, six tips (8.8%) had positive cultures. The catheter tip was collected for semiquantitative and qualitative microbiological analysis. From November 2011 to April 2012, patients electively operated and maintained under epidural catheter for postoperative analgesia were evaluated. The increased use of epidural analgesia with catheter leads to the need to demonstrate the safety of this method and know the incidence of catheter colonization, inserted postoperatively for epidural analgesia, and the bacteria responsible for this colonization. Stabille, Débora Miranda Diogo Filho, Augusto Diogo Mandim, Beatriz Lemos da Silva Araújo, Lúcio Borges de Mesquita, Priscila Miranda Diogo Jorge, Miguel Tanús Postoperative epidural catheter analgesia, under these study conditions, was found to be low risk for bacterial colonization in patients at surgical wards. Stabille, Débora Miranda Diogo Diogo Filho, Augusto Mandim, Beatriz Lemos da Silva de Araújo, Lúcio Borges Mesquita, Priscila Miranda Diogo Jorge, Miguel Tanús
Refrigeration of CVC tips sent for culture and culturing only those from patients with positive blood cultures reduce the workload in the microbiology laboratory without misdiagnosing CR-BSI.įrequency of colonization and isolated bacteria from the tip of epidural catheter implanted for postoperative analgesia. Only 61 CVCs were obtained from patients with CR-BSI, and in most of them, blood cultures were already positive before CVC culture, so only 0.91% of the CR-BSI episodes would have been misdiagnosed as culture negative after refrigeration. The difference showed that 6-day refrigeration reduced the number of significant CVCs by 15.2%. The yield of refrigerated CVC tips that grow significant colony counts of primary culture in group B was compared with the yield of refrigerated catheter tips in group A. In group A, the catheters were recultured after 24 h of refrigeration, and in group B, the catheters were recultured after 6 days more of refrigeration, so that the refrigeration time evaluated would be of 6 days. We selected all the catheters with a significant count according to the Maki's roll-plate technique and randomly assigned them to 2 groups. We evaluated the effect of 6-day refrigeration of 215 CVC tips. Our working hypothesis was that CVC tips should be refrigerated and cultured only in patients with positive blood cultures. Moreover, CVC tip culture increases laboratory workload, and reports of colonization may be meaningless or misleading for the clinician. However, a high proportion of these suspicions are not confirmed. Present guidelines recommend culturing only central venous catheter (CVC) tips from patients with suspected catheter-related bloodstream infection (CR-BSI). Are central venous catheter tip cultures reliable after 6-day refrigeration?īouza, Emilio Guembe, Maria Gómez, Haydee MartÃn-Rabadán, Pablo Rivera, Marisa Alcalá, Luis
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